The Alexa Fluor series of fluorescent dyes, as commonly used dyes in visible spectra, is a major breakthrough in fluorescence labeling reagents. The combination of R-PE or APC with Tandem dyes has expanded the application of Alexa Fluor in multicolor detection.

Alexa Fluor is a series of fluorescent dyes that have been used in recent years and are very suitable for laser confocal focusing. Its characteristic is that the excitation peak is suitable for the filter of the laser, and the emission peak is relatively narrow, which is conducive to various fluorescent labeling and has strong quenching resistance.

Advantages of Alexa Fluor Fluorescent Dyes:

Brightness - Alexa Fluor conjugates exhibit stronger fluorescence compared to other dyes.

Photostability - Alexa Fluor conjugates have stronger photostability compared to other dyes, providing longer image capture time.

Instrument compatibility - The absorption spectrum of Alexa Fluor coupling compound matches the main output wavelength of a regular excitation light source.

Multiple colors - Alexa Fluor conjugates exhibit various direct fluorescence from blue to red

PH insensitivity - Alexa Fluor dyes maintain strong fluorescence over a wide pH range

Water solubility - Alexa Fluor reactive dyes have good water solubility, can bind to proteins without the need for organic reagents, and are less prone to precipitation during storage

Commonly used include:

Alexa 488 can be excited by an argon ion laser with a 488nm filter laser, emitting green light that is similar in wavelength to GFP, FITC, and CY2;

Alexa 633 can be excited using a 633nm filter, with a wavelength similar to Cy5.